The role of deuterium in molecular evolution
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rium also affects the stability and geometry of hydrogen bonds in apparently rather complex way and may, through the changes in the hydrogen bond zero-point vibrational energies, alter the conformational dynamics of hydrogen (deuterium)-bonded structures within the DNA and protein in 2H2O.
5. CONCLUSION
The successful adaptation of organisms to high concentration of 2H2O will open a new avenues of investigation with using [U- 2H]labeled macromolecules could be isolated from these organisms. For example, fully deuterated essential macromolecules as proteins and nucleic acids will give promise of important biological, medical and diagnostical uses. Modern physical methods of study the structure of [U- 2H]labeled macromolecules, particularly three-dimentional NMR in a combination with crystallography methods, X-ray diffraction, IR-, and CD- spectroscopy should cast new light on many obscure problems concerning with the biological introduction of deuterium into molecules of DNA and proteins as well as the structure and the function of macromolecules in the presence of 2H2O. The variety of these and other aspects of biophysical properties of fully deuterated macromolecules in the presence of 2H2O remain an interesting task for the future.
First, I hope that the structural and the functional studies of [U- 2H]labeled macromolecules can provide us to the useful information about a many aspects of the synthesis of fully deuterated macromolecules and their biophysical behaviour in 2H2O.
Second, the extensive body of available structural data about a cell protection system (at the level of the structure and the functioning of [U- 2H]labeled DNA and enzymes) will also form the basis for a particularly useful model for the study of biological adaptation to 2H2O in aspect of molecular evolution of macromolecules with difined isotopic structures.
Finally, we also believe, the research can make a favour the medicine and biotechnology, especially for creating a fully deuterated analogues of enzymes and DNA having something different properties then the protonated species and working in the presence of 2H2O.
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